Tissue-specific differences in the spatial interposition of X-chromosome and 3R chromosome regions in the malaria mosquito Anopheles messeae fall

doi:10.1371/journal.pone.0115281

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Tissue-specific differences in the spatial interposition of X-chromosome and 3R chromosome regions in the malaria mosquito Anopheles messeae fall	6 MB	Adobe Acrobat PDF	Read	Download
DOI Доступ к ресурсу на сайте издателя			10.1371/journal.pone.0115281

#малярийные комары

#пространственная организация хромосом

#X-хромосомы

#нурзальные клетки

Title: Tissue-specific differences in the spatial interposition of X-chromosome and 3R chromosome regions in the malaria mosquito Anopheles messeae fall
Creator: Artemov, Gleb N.
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Sapunov, Gleb
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Stegniy, Vladimir N.
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Bondarenko, Semen
Date: 2015
DOI: 10.1371/journal.pone.0115281
Description: Spatial organization of a chromosome in a nucleus is very important in biology but many aspects of it are still generally unresolved. We focused on tissue-specific features of chromosome architecture in closely related malaria mosquitoes, which have essential inter-specific differences in polytene chromosome attachments in nurse cells. We showed that the region responsible for X-chromosome attachment interacts with nuclear lamina stronger in nurse cells, then in salivary glands cells in Anopheles messeae Fall. The inter-tissue differences were demonstrated more convincingly in an experiment of two distinct chromosomes interposition in the nucleus space of cells from four tissues. Microdissected DNA-probes from nurse cells X-chromosome (2BC) and 3R chromosomes (32D) attachment regions were hybridized with intact nuclei of nurse cells, salivary gland cells, follicle epithelium cells and imaginal disсs cells in 3D-FISH experiments. We showed that only salivary gland cells and follicle epithelium cells have no statistical differences in the interposition of 2BC and 32D. Generally, the X-chromosome and 3R chromosome are located closer to each other in cells of the somatic system in comparison with nurse cells on average. The imaginal disсs cell nuclei have an intermediate arrangement of chromosome interposition, similar to other somatic cells and nurse cells. In spite of species-specific chromosome attachments there are no differences in interposition of nurse cells chromosomes in An. messeae and An. atroparvus Thiel. Nurse cells have an unusual chromosome arrangement without a chromocenter, which could be due to the special mission of generative system cells in ontogenesis and evolution.

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collection(s): Институт биологии, экологии, почвоведения, сельского и лесного хозяйства (Биологический институт) | НИИ биологии и биофизики
Identifier: смотреть в электронном каталоге НБ ТГУ
Type: статьи в журналах
Source: PLoS ONE. 2015. Vol. 10, № 2. P. e0115281 (1-14)
Language: eng

Created: 16-04-2018

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Институт биологии, экологии, почвоведения, сельского и лесного хозяйства (Биологический институт)

НИИ биологии и биофизики Tissue-specific differences in the spatial interposition of X-chromosome and 3R chromosome regions in the malaria mosquito Anopheles messeae fall