https://vital.lib.tsu.ru/vital/access/manager/Index ${session.getAttribute("locale")} 5 Isolation, characterization, and metal response of novel, acid-tolerant Penicillium spp. from extremely metal-rich waters at a mining site in Transbaikal (Siberia, Russia) https://vital.lib.tsu.ru/vital/access/manager/Repository/vtls:000724006  2 g l-1 each of Cu, Zn, Al, and As. Both isolates were identified as Penicillium spp. by phylogenetic analyses and they grew well in Czapek medium acidified to pH 2.5. Resistance to Cu, Cd, Ni, Co, and arsenate was in the range of 1-10 g l-1. Further experiments with Penicillium strain ShG4C demonstrated that growth in Cu-containing media was accompanied by the precipitation of Cu-oxalate (moolooite) and the formation of extracellular vesicles enriched in Cu on the mycelia. Vesicles were greatly reduced in size in Cd-containing media and were not formed in the presence of Ni or Co. Cd-oxalate was detected as a crystalline solid phase in Cd-exposed mycelia. Hydrated Ni-sulfate (retgersite) and Co-sulfate (bieberite) were detected in mycelia grown in the presence of Ni and Co, respectively. The results demonstrated that acid-tolerant and metal-resistant Penicillium constitute a component in extremophilic microbiomes, contributing to organic matter breakdown and formation of secondary solid phases at pH ranges found in acid rock drainage.]]> Tue 22 Sep 2020 09:37:20 KRAT ]]> A metagenomic window into the 2-km-deep terrestrial subsurface aquifer revealed multiple pathways of organic matter decomposition https://vital.lib.tsu.ru/vital/access/manager/Repository/vtls:000651032 Mon 01 Apr 2019 16:57:20 KRAT ]]>