Two-photon microscopy methods have been actively developing in the last two decades. In particular, various approaches are being developed to analyze metabolic activity obtained by laser microscopy with high temporal resolution. One such approach is ploting the autofluorescence lifetime signal data to the phasor plot and observe deviations from the normal state. This tool has proven itself well for analyzing the metabolic activity of biological objects, including bacteria. This study analyzes the antimicrobial activity for MRSA bacteria by phasor plot approach. It was possible to show that, the lifetime of autofluorescence in the phasor plane changes depending on the type of nanoparticles used, with which bacteria are incubated. This result can be used in the future for rapid assessment of the antimicrobial activity of nanoparticles. The research was carried out with the support of a grant under the Decree of the Government of the Russian Federation No. 220 of 09 April 2010 (Agreement No. 075-15-2021-615 of 04 June 2021) and was performed according to the Government research assignment for ISPMS SB RAS, project FWRW-2019-0033
Proceedings of SPIE. 2021. Vol. 12086 : XV International Conference on Pulsed Lasers and Laser Applications, 2021, Tomsk, Russian Federation. P. 120861J-1-120861J-6
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Efficiency of the phasor plot approach for the analysis of the antimicrobial properties of nanoparticles using two-photon microscopy