In this study, the targeted bisulfite massive parallel sequencing was used to study the level of gene methylation (ANKRD53, GATA3, CALCB, TRPV6, SLC13A4) in miscarriages. Using bioinformatics approaches, we found a statistically significant increase in methylation in the promoter regions of analyzed genes. A relationship was observed between the level of methylation of the ANKRD53 gene, which is responsible for chromosome segregation in mitotic division and the fraction of cells with a trisomy karyotype of miscarriages with aneuploidy. Also, in this sample, aberrant methylation of the regulatory region of the transcription factor GATA3 was noted, which is the main component for the timely differentiation of cytotrophoblast cells in the prenatal period. The promoters of the remaining genes (CALCB, TRPV6 and SLC13A4) were also hypermethylated, which may affect the outcome of embryo development in the early stages ofpregnancy.
Перспективы развития фундаментальных наук : сборник научных трудов XVII Международной конференции студентов, аспирантов и молодых ученых, Россия, Томск, 21-24 апреля 2020 г.. Томск, 2020. Т. 4 : Биология и фундаментальная медицина. С. 76-78
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