Extraction and preconcentration of the highly lipophilic antioxidant 2,6-Diisobornyl-4-Methylphenol and its metabolite from plasma samples for the determination by HPLC–MS/MS

doi:10.1134/S1061934823020065

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Extraction and preconcentration of the highly lipophilic antioxidant 2,6-Diisobornyl-4-Methylphenol and its metabolite from plasma samples for the determination by HPLC–MS/MS	1 MB	Adobe Acrobat PDF	Read	Download
DOI Доступ к ресурсу на сайте издателя			10.1134/S1061934823020065

#подготовка образцов плазмы

#диборнол

#простагенин

#фенольные антиоксиданты

#метод жидкостной хроматографии и тандемной масс-спектрометрии

Title: Extraction and preconcentration of the highly lipophilic antioxidant 2,6-Diisobornyl-4-Methylphenol and its metabolite from plasma samples for the determination by HPLC–MS/MS
Creator: Lakeev, Alexander P.
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Yanovskaya, Elena A.
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Yanovsky, Vyacheslav A.
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Andropov, Mikhail O.
Date: 2023
DOI: 10.1134/S1061934823020065
Description: A procedure is proposed for the extraction of 2,6-diisobornyl-4-methylphenol (IBP), the molecule with potential multi-target activity, and its active metabolite 2,6-diisobornyl-4-hydroxymethylphenol (IBP–OH) from human and rat plasma samples for further determination by liquid chromatography–tandem mass spectrometry. The solubility of IBP (in mg/mL) in various organic solvents is determined. In particular, the compound is readily soluble in chloroform (346 ± 14), methyl tert-butyl ether (98.6 ± 3.8), and ethyl acetate (96.8 ± 2.0). It is shown that the use of a mixture of chloroform and isopropanol (5 : 1, v/v) as an extraction system with simultaneous protein precipitation makes it possible to extract only 66 ± 10% of IBP, while the recovery of IBP–OH is 96 ± 7%. Pre-dilution of 200 µL of plasma with 300 µL saline increases IBP recovery to 100 ± 4%. Drying the extracts in a vacuum concentrator (air, 45°C) with the further reconstitution of the residues in 300 μL of acetonitrile facilitates the purification of biosamples and the preconcentration of analytes. The total sample preparation time does not exceed 70 min, and the duration of an analysis is 7 min, the injection volume is 2 µL. The procedure has been successfully tested on actual rat plasma samples after a single oral administration of IBP (10 mg/kg in oil) in the framework of pharmacokinetic studies.

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collection(s): Химический факультет
Identifier: смотреть в электронном каталоге НБ ТГУ
Type: статьи в журналах
Source: Journal of analytical chemistry. 2023. Vol. 78, № 2. P. 231-240
Language: eng

Created: 06-03-2025

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Химический факультет Extraction and preconcentration of the highly lipophilic antioxidant 2,6-Diisobornyl-4-Methylphenol and its metabolite from plasma samples for the determination by HPLC–MS/MS