Physical mapping of the Anopheles (Nyssorhynchus) darlingi genomic scaffolds

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Physical mapping of the Anopheles (Nyssorhynchus) darlingi genomic scaffolds	3 MB	Adobe Acrobat PDF	Read	Download
DOI Доступ к ресурсу на сайте издателя			10.3390/insects12020164

Title: Physical mapping of the Anopheles (Nyssorhynchus) darlingi genomic scaffolds
Creator: Rafael, Míriam Silva
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Bridi, Leticia Cegatti
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Sharakhov, Igor V.
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Marinotti, Osvaldo
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Sharakhova, Maria V.
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Timoshevskiy, Vladimir A.
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Guimarães-Marques, Giselle Moura
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Santos, Valéria Silva
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Silva, Carlos Gustavo Nunes da
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Astolfi-Filho, Spartaco
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Tadei, Wanderli Pedro
Date: 2021
DOI: 10.3390/insects12020164
Description: Anopheles darlingi mosquitoes are the main vectors of malaria in the Brazilian Amazon. To assign genomic DNA sequences to chromosomes of this species, we performed fluorescence in situ hybridization of DNA probes with salivary glands polytene chromosomes. We compared the physical locations of the An. darlingi probes with homologous sequences in other Anopheles species, namely Anopheles albimanus and Anopheles gambiae. The results demonstrated that substantial genome rearrangements occurred throughout the evolutionary history of these mosquitoes. The physical mapping data can be useful for improving the structural accuracy of the An. darlingi genome assembly and for understanding the chromosomal evolution of these mosquitoes