The paper presents the results of testing the properties of antibacterial nonwoven fibrous materials based on polylactic acid with incorporated chloramphenicol. It has been shown that exposure to a pulsed electron beam increased the rate of release of chloramphenicol into a phosphate-buffered saline medium. In this case, the amount of the substance released from treated samples was reliably greater than that with untreated samples during the five days of the experiment.