Quantitative imaging of white and gray matter remyelination in the cuprizone demyelination model using the macromolecular proton fraction

doi:10.3390/cells8101204

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Quantitative imaging of white and gray matter remyelination in the cuprizone demyelination model using the macromolecular proton fraction	3 MB	Adobe Acrobat PDF	Read	Download
DOI Доступ к ресурсу на сайте издателя			10.3390/cells8101204

#магнитно-резонансная томография

#олигодендроциты

#иммуногистохимия

#клетки-предшественники олигодендроцитов

#ремиелинизация

#демиелинизация

#купризоновая модель

#макромолекулярная протонная фракция

#миелин

Title: Quantitative imaging of white and gray matter remyelination in the cuprizone demyelination model using the macromolecular proton fraction
Creator: Pishchelko, Anna O.
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Glazacheva, Valentina Y.
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Pan, Edgar S.
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Akulov, Andrey E.
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Svetlik, Mikhail V.
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Tyumentseva, Yana
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Ananina, Tatyana V.
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Yarnykh, Vasily L.
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Khodanovich, Marina Yu.
Date: 2019
DOI: 10.3390/cells8101204
Description: Macromolecular proton fraction (MPF) has been established as a quantitative clinically-targeted MRI myelin biomarker based on recent demyelination studies. This study aimed to assess the capability of MPF to quantify remyelination using the murine cuprizone-induced reversible demyelination model. MPF was measured in vivo using the fast single-point method in three animal groups (control, cuprizone-induced demyelination, and remyelination after cuprizone withdrawal) and compared to quantitative immunohistochemistry for myelin basic protein (MBP), myelinating oligodendrocytes (CNP-positive cells), and oligodendrocyte precursor cells (OPC, NG2-positive cells) in the corpus callosum, caudate putamen, hippocampus, and cortex. In the demyelination group, MPF, MBP-stained area, and oligodendrocyte count were significantly reduced, while OPC count was significantly increased as compared to both control and remyelination groups in all anatomic structures (p < 0.05). All variables were similar in the control and remyelination groups. MPF and MBP-stained area strongly correlated in each anatomic structure (Pearson's correlation coefficients, r = 0.80-0.90, p < 0.001). MPF and MBP correlated positively with oligodendrocyte count (r = 0.70-0.84, p < 0.01 for MPF; r = 0.81-0.92, p < 0.001 for MBP) and negatively with OPC count (r = -0.69--0.77, p < 0.01 for MPF; r = -0.72--0.89, p < 0.01 for MBP). This study provides immunohistological validation of fast MPF mapping as a non-invasive tool for quantitative assessment of de- and remyelination in white and gray matter and indicates the feasibility of using MPF as a surrogate marker of reparative processes in demyelinating diseases.