Revealed that the distribution of spots on the surface of the concrete depends on its density and structure, and the penetration of oil into the concrete occurs through micro- and nano-pores and cracks of the further spread of spots along the pathways. It was found that the use of hydrocarbon-oxidizing microorganisms strains as Micrococcus luteus IS16, M. varians IS41, micromycetes: Aspergillus sp., Penicillium sp., Alternaria sp., thiobacteria Acidithiobacillus ferrooxidans Ach1, A. ferrooxidans Ach2 promising for biological treatment of oil-contaminated concrete. Create optimal conditions for maintaining the activity of microorganisms: 55,0 ± 15,0% moisture, nutrients, temperature 38,0 ± 2,00C, trace elements, optimize the biodegradation of petroleum hydrocarbons.