Development of DNA aptamers to native EpCAM for isolation of lung circulating tumor cells from human blood

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Development of DNA aptamers to native EpCAM for isolation of lung circulating tumor cells from human blood	6 MB	Adobe Acrobat PDF	Read	Download
DOI Доступ к ресурсу на сайте издателя			10.3390/cancers11030351

Title: Development of DNA aptamers to native EpCAM for isolation of lung circulating tumor cells from human blood
Creator: Kolovskaya, Olga S.
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Ivanchenko, Tatiana I.
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Zamay, Tatiana N.
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Veprintsev, Dmitry V.
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Grigorieva, Valentina L.
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Garanzha, Irina I.
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Krat, Alexey V.
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Glazyrin, Yury E.
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Gargaun, Ana
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Zamay, Galina S.
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Lapin, Ivan N.
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Svetlichnyi, Valerii A.
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Berezovski, Maxim V.
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Kichkailo, Anna S.
Date: 2019
DOI: 10.3390/cancers11030351
Description: We selected DNA aptamers to the epithelial cell adhesion molecule (EpCAM) expressed on primary lung cancer cells isolated from the tumors of patients with non-small cell lung cancer using competitive displacement of aptamers from EpCAM by a corresponding antibody. The resulting aptamers clones showed good nanomolar affinity to EpCAM-positive lung cancer cells. Confocal microscopy imaging and spectral profiling of lung cancer tissues confirmed the same protein target for the aptamers and anti-EpCAM antibodies. Furthermore, the resulted aptamers were successfully applied for isolation and detection of circulating tumor cells in clinical samples of peripheral blood of lung cancer patients.