Hemolysis and ATP release from human and rat erythrocytes under conditions of hypoxia: a comparative study

doi:10.7868/S0233475518010036

Эндаумент фонд ТГУ!
Впиши своё имя в историю университета
- Сделать пожертвование -

Add to "Quick Collection"

Description	Size	Format
Hemolysis and atp release from human and rat erythrocytes under conditions of hypoxia: a comparative study	677 KB	Adobe Acrobat PDF	Read	Download
DOI Доступ к ресурсу на сайте издателя			10.7868/S0233475518010036

#гемолиз

#эритроциты

#аденозинтрифосфорная кислота

#молекулярные механизмы

#экто-АТФаза

Title: Hemolysis and ATP release from human and rat erythrocytes under conditions of hypoxia: a comparative study
Creator: Luneva, Oksana G.
|
Novozhilova, T. S.
|
Alekseeva, Natalya V.
|
Rodnenkov, Oleg V.
|
Deev, Leonid I.
|
Maksimov, Georgy V.
|
Grygorczyk, Ryszard
|
Orlov, Sergei N.
|
Sidorenko, Svetlana V.
Date: 2018
DOI: 10.7868/S0233475518010036
Description: Red blood cells are involved not only in transportation of oxygen and carbon dioxide but also in autoregulation of vascular tone by ATP release in hypoxic conditions. Molecular mechanisms of the ATP release from red blood cells in response to a decrease in partial oxygen pressure still remain to be elucidated. In this work we have studied effects of hypoxia on red blood cell hemolysis in humans and rats and compared the effects of inhibitors of ecto-ATPase and pannexin on the release of ATP and hemoglobin from rat erythrocytes. The 20-min hypoxia at 37°C increased hemolysis of red blood cells in humans and rats 1.5- and 2.5-fold, respectively. In rat erythrocytes a significant increase in hypoxia-induced extracellular ATP level was found only in the presence of ecto-ATPase inhibitor ARL 67156. In these conditions we observed a positive correlation (R2 = 0.5003) between the increase in free hemoglobin concentration and the ATP release. Neither carbenoxolon nor probenecid, the inhibitors of low-selectivity pannexin channels, altered the hypoxia-induced ATP release from rat erythrocytes. The obtained results indicate a key role of hemolysis in the ATP release from red blood cells.