Clinically relevant morphological structures in breast cancer represent transcriptionally distinct tumor cell populations with varied degrees of epithelial-mesenchymal transition and CD44+CD24- stemness

doi:10.18632/oncotarget.18022

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Clinically relevant morphological structures in breast cancer represent transcriptionally distinct tumor cell populations with varied degrees of epithelial-mesenchymal transition and CD44+CD24- stemness	5 MB	Adobe Acrobat PDF	Read	Download
DOI Доступ к ресурсу на сайте издателя			10.18632/oncotarget.18022

#рак молочной железы

#опухолевые клетки

#хромосомные аберрации

#внутриутробная морфологическая гетерогенность

#эпителиально-мезенхимальный переход

Title: Clinically relevant morphological structures in breast cancer represent transcriptionally distinct tumor cell populations with varied degrees of epithelial-mesenchymal transition and CD44+CD24- stemness
Creator: Skryabin, Nikolay A.
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Gerashchenko, Tatiana S.
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Tashireva, Liubov A.
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Wilhelm, Jochen
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Buldakov, Mikhail A.
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Sleptcov, Aleksei A.
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Lebedev, Igor N.
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Vtorushin, Sergey V.
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Zavyalova, Marina V.
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Denisov, Evgeny V.
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Cherdyntseva, Nadezhda V.
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Perelmuter, Vladimir M.
Date: 2017
DOI: 10.18632/oncotarget.18022
Description: Intratumor morphological heterogeneity in breast cancer is represented by different morphological structures (tubular, alveolar, solid, trabecular, and discrete) and contributes to poor prognosis; however, the mechanisms involved remain unclear. In this study, we performed 3D imaging, laser microdissection-assisted array comparative genomic hybridization and gene expression microarray analysis of different morphological structures and examined their association with the standard immunohistochemistry scorings and CD44+CD24- cancer stem cells. We found that the intratumor morphological heterogeneity is not associated with chromosomal aberrations. By contrast, morphological structures were characterized by specific gene expression profiles and signaling pathways and significantly differed in progesterone receptor and Ki-67 expression. Most importantly, we observed significant differences between structures in the number of expressed genes of the epithelial and mesenchymal phenotypes and the association with cancer invasion pathways. Tubular (tube-shaped) and alveolar (spheroid-shaped) structures were transcriptionally similar and demonstrated co-expression of epithelial and mesenchymal markers. Solid (large shapeless) structures retained epithelial features but demonstrated an increase in mesenchymal traits and collective cell migration hallmarks. Mesenchymal genes and cancer invasion pathways, as well as Ki-67 expression, were enriched in trabecular (one/two rows of tumor cells) and discrete groups (single cells and/or arrangements of 2-5 cells). Surprisingly, the number of CD44+CD24- cells was found to be the lowest in discrete groups and the highest in alveolar and solid structures. Overall, our findings indicate the association of intratumor morphological heterogeneity in breast cancer with the epithelial-mesenchymal transition and CD44+CD24- stemness and the appeal of this heterogeneity as a model for the study of cancer invasion.

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collection(s): НИИ биологии и биофизики
Identifier: смотреть в электронном каталоге НБ ТГУ
Relation: Oncotarget
Type: статьи в журналах
Source: Oncotarget. 2017. Vol. 8, № 37. P. 61163-61180
Language: eng

Created: 24-09-2018

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НИИ биологии и биофизики Clinically relevant morphological structures in breast cancer represent transcriptionally distinct tumor cell populations with varied degrees of epithelial-mesenchymal transition and CD44+CD24- stemness