Fluorescent angiography of chicken embryo and photobleaching velocimetry

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DOI Доступ к ресурсу на сайте издателя			10.1117/12.2267948

Title: Fluorescent angiography of chicken embryo and photobleaching velocimetry
Creator: Namykin, Anton A.
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Fedosov, Ivan V.
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Postnov, Dmitry E.
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Tuchin, Valery V.
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Stiukhina, Elena S.
Date: 2017
DOI: 10.1117/12.2267948
Description: Fluorescent angiography approach in application to a living chicken embryo is discussed. It provides precise vessel wall detection and demonstrates usefulness for real time monitoring of vasoconstriction and vasodilatation related to self regulation of vascular network as well as to response to external factors. On the other hand, high stability of fluorescence and long period of dye elimination makes variations of fluorescent intensity practically independent from fast variations of blood flow rate. Therefore, we proposed the improvement of fluorescent angiography technique by introduction of photobleaching fluorescent velocimetry approach. We have developed the imaging system for intravital microscopic photobleaching velocimetry and tested it by using a glass capillary tube as a model of blood vessel. We demonstrated high potential of the technique for instant flow velocity distribution profile measurement with high spatial and temporal resolution up to 2 μm and 60 ms, respectively.

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collection(s): Научное управление
Identifier: смотреть в электронном каталоге НБ ТГУ
Type: статьи в журналах
Source: Proceedings of SPIE. 2017. Vol. 10336 : Saratov Fall Meeting 2016 : Optical Technologies in Biophysics and Medicine XVIII, 27-30 September 2016, Saratov, Russian Federation. P. 103360V-1-103360V-9
Language: eng

Created: 22-12-2017

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Научное управление Fluorescent angiography of chicken embryo and photobleaching velocimetry