Mosquitoes of Anopheles hyrcanus (Diptera, Culicidae) group: species diagnostic and phylogenetic relationships

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Mosquitoes of Anopheles hyrcanus (Diptera, Culicidae) group: species diagnostic and phylogenetic relationships	465 KB	Adobe Acrobat PDF	Read	Download
DOI Доступ к ресурсу на сайте издателя			10.4269/ajtmh.14-0207

Title: Mosquitoes of Anopheles hyrcanus (Diptera, Culicidae) group: species diagnostic and phylogenetic relationships
Creator: Khrabrova, Natalia V.
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Sibataev, Anuarbek K.
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Alekseeva, Svetlana S.
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Esenbekova, Perizat A.
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Andreeva, Yulia V.
Date: 2015
DOI: 10.4269/ajtmh.14-0207
Description: Herein, we report the results of study of Anopheles species in Primorsk and Khabarovsk regions of Russia. Three species of the Anopheles hyrcanus group: An. kleini, An. pullus, and An. lesteri were identified by molecular taxonomic diagnostics for the first time in Russia. Surprisingly, An. sinensis, which earlier was considered the only species of Anopheles in Russian Far East, was not observed. We analyzed nucleotide variation in the 610-bp fragment of the 5' end of the cytochrome c oxidase subunit I (COI) region. All species possessed a distinctive set of COI sequences. A maximum likelihood phylogenetic tree was constructed for members of the hyrcanus group. The examined Anopheles hyrcanus group members could be divided into two major subgroups: subgroup 1 (An. hyrcanus and An. pullus) and subgroup 2 (An. sinensis, An. kleini, and An. lesteri), which were found to be monophyletic.