The influence of organic solvent composition on the extraction efficiency of medium- and long-chain fatty acids from human feces flurries has been investigated. To this end we tested the following organic solvents: hexane, methyl tertiary-butyl ether and their mixture in a ratio 1:1. Furthermore, we monitored the effects of ionic strength and pH of the flurries on the recovery of fatty acids. A high-performance liquid chromatography mass spectrometry (HPLS-MS) method was used for monitoring of the fatty acids in the obtained extracts. We report that within the tested conditions the most optimal combination of a recovery and analytical coverage of fatty acids was obtained with flurries containing 4% HCl and 5.5% NaCl and a mixture of methyl tertiary-butyl ether and hexane as an organic phase.
Перспективы развития фундаментальных наук : сборник научных трудов XX Международной конференции студентов, аспирантов и молодых ученых, 25–28 апреля 2023 г.. Томск, 2023. Т. 2 : Химия. С. 190-192