The present study was aimed to improve in vitro shoot proliferation of octoploid strawberry cultivar ‘Solnechnaya polyanka’ on different nutrient media (MS, B5 or MS+B5), supplemented with various concentrations (2.0, 4.0, 8.0 ȝM) of thidiazuron (TDZ). The best regeneration frequency (100%) with the highest rate of strawberry axillary shoot proliferation (14.1 ± 1.24 shoots per explant) and maximum shoot length (1.92 ± 0.04 cm) were achieved using precultivation of explants for 3 days on induction media B5 supplemented with 4.0 ȝM TDZ followed by cultivation of regenerants on hormone-free media B5 for 6 weeks.
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