A standard photomap of ovarian nurse cell chromosomes and inversion polymorphism in Anopheles beklemishevi

doi:10.1186/s13071-018-2657-3

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A standard photomap of ovarian nurse cell chromosomes and inversion polymorphism in Anopheles beklemishevi	2 MB	Adobe Acrobat PDF	Read	Download
DOI Доступ к ресурсу на сайте издателя			10.1186/s13071-018-2657-3

#цитогенетическая карта

#инверсионный полиморфизм

#малярийные комары

#комары

Title: A standard photomap of ovarian nurse cell chromosomes and inversion polymorphism in Anopheles beklemishevi
Creator: Gordeev, Mikhail I.
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Kokhanenko, Alina A.
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Moskaev, Anton V.
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Velichevskaya, Alena I.
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Stegniy, Vladimir N.
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Sharakhov, Igor V.
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Sharakhova, Maria V.
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Artemov, Gleb N.
Date: 2018
DOI: 10.1186/s13071-018-2657-3
Description: Background Anopheles beklemishevi is a member of the Maculipennis group of malaria mosquitoes that has the most northern distribution among other members of the group. Although a cytogenetic map for the larval salivary gland chromosomes of this species has been developed, a high-quality standard cytogenetic photomap that enables genomics and population genetics studies of this mosquito at the adult stage is still lacking. Methods In this study, a cytogenetic map for the polytene chromosomes of An. beklemishevi from ovarian nurse cells was developed using high-resolution digital imaging from field collected mosquitoes. PCR-amplified DNA probes for fluorescence in situ hybridization (FISH) were designed based on the genome of An. atroparvus. The DNA probe obtained by microdissection procedures from the breakpoint region was labelled in a DOP-PCR reaction. Population analysis was performed on 371 specimens collected in 18 locations. Results We report the development of a high-quality standard photomap for the polytene chromosomes from ovarian nurse cells of An. beklemishevi. To confirm the suitability of the map for physical mapping, several PCR-amplified probes were mapped to the chromosomes of An. beklemishevi using FISH. In addition, we identified and mapped DNA probes to flanking regions of the breakpoints of two inversions on chromosome X of this species. Inversion polymorphism was determined in 13 geographically distant populations of An. beklemishevi. Four polymorphic inversions were detected. The positions of common chromosomal inversions were indicated on the map. Conclusions The study constructed a standard photomap for ovarian nurse cell chromosomes of An. beklemishevi and tested its suitability for physical genome mapping and population studies. Cytogenetic analysis determined inversion polymorphism in natural populations of An. beklemishevi related to this species’ adaptation