It is known that the violation of DNA methylation processes is one of the earliest and most common events in malignant tumors. The purpose of this study was to perform a comparative analysis of the methylation level of LINE-1 retroelements in blood cirDNA in patients with lung cancer before treatment and at the stages of dynamic observation after antitumor therapy. The study included 16 patients with non-small cell lung cancer (NSCLC). The material for the study was venous blood, which was taken before the treatment (10-15 days before the start of treatment), 15-30 days after the end of the last course of non-adjuvant chemotherapy and in the postoperative period for 10-15 days. Analysis of the methylation level of LINE-1 element was performed using a quantitative methyl specific PCR .A linear increase in the methylation index of the LINE-1 fragment in response to antitumor therapy was revealed: after chemotherapy, a statistically significant increase in the methylation index was approximately 2-fold (p = 0.04, paired t-test), 1.4 times after operation indicators after chemotherapy (p = 0.134, paired t-test). As a result, after combined treatment, the LINE-1 methylation index in cc-cirDNA increased 3-fold compared to the level before treatment (p = 0.03, paired t-test).
Перспективы развития фундаментальных наук : сборник научных трудов XV Международной конференции студентов, аспирантов и молодых ученых, Россия, Томск, 24-27 апреля 2018 г.. Томск, 2018. Т. 4 : Биология и фундаментальная медицина. С. 155-157
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Циркулирующие ДНК-маркеры при мониторинге рака легкого: анализ статуса метилирования ретроэлементов LINE-1