The study was conducted during 2004–2015 in West Siberia natural forests. The Tomsk region mycobiota is almost unstudied though this region is considered to be an accumulation zone for North American, European, and Far East fungi species. Sampling was implemented in pure cedar or pine stands. Molecular data and microscoping identified 283 macromycetes species, which form ectomycorrhizas with Pinus sibirica and Pinus sylvestris.